- Ultramorphological Assessment of Dentin-Resin Interface After Use of Simplified Adhesives.
Ultramorphological Assessment of Dentin-Resin Interface After Use of Simplified Adhesives.
Oper Dent. 2014 Oct 9;
Authors: Marghalani H, Bakhsh T, Sadr A, Tagami J
SUMMARY This study assessed dentin/resin interface integration in Class I cavities restored with simplified adhesives by using a focused ion-beam milling (FIB) and transmission electron microscope (TEM). Class I cavities (1.5-mm depth with dentin thickness of ∼0.5 mm, 4-mm length, and 2-mm width) were prepared on freshly extracted, sound human molars. Two all-in-one adhesive systems (Scotchbond/Single Bond Universal [SUD] and Xeno-V(+) [X5D]) were used and compared with a two-step etch-and-rinse system (Prime&Bond NT [NTD]). The adhesives were applied according to the manufacturers’ guidelines. A universal resin composite (Filtek Z350 XT Universal) was used to restore the cavities in one bulk filling and was irradiated at 550 mW/cm(2) for 40 seconds by a quartz-tungsten-halogen light (Optilux 501). After exposure to liquid nitrogen coolant, the specimens were milled to nanoscale thickness by FIB to view and then assess the area of dentin-resin interface by TEM. Unlike the unfilled X5D, a noticeably smooth transition zone at the dentin-resin interface was shown for the SUD and NTD adhesives. The SUD demonstrated an uneven hybrid layer with clearly demineralized collagen bundles. Ultramorphologically, dispersed needlelike apatite crystals were detected within the partially demineralized dentin or the hybrid layer of both compositionally different all-in-one simplified adhesives. Conversely, these crystals were entirely absent from the hybrid layer of the etch-and-rinse NTD adhesive. In the X5D group, a bright band was noted beneath the hybrid layer. The methacryloxydecyl dihydrogen phosphate monomer containing ultramild self-etch adhesive (SUD) was still validated in terms of its capability in dentin adhesion.
( 25299704)- as supplied by publisher]
- The effect of tooth age on color adjustment potential of resin composite restorations.
The effect of tooth age on color adjustment potential of resin composite restorations.
J Dent. 2014 Sep 18;
Authors: Tanaka A, Nakajima M, Seki N, Foxton R, Tagami J
OBJECTIVES: The purpose of this study was to investigate the effect of tooth age on color adjustment potential of resin composite restorations in human teeth.
METHODS: Twenty extracted human premolars with an A2 shade, extracted for orthodontic reasons from younger patients (20-28 yrs) (younger teeth) and periodontal reasons from older patients (45-69 yrs) (older teeth), were used in this study. Cylindrical shaped cavities (3.0mm depth; 2.0mm diameter) were prepared in the center of the crowns on the buccal surface. One of four resin composites of A2 shade (Kalore, KA; Solare, SO; Clearfil Majesty, MJ; Beautifil II, BF) was placed in the cavity, and the color was measured at four areas (0.4mm x 0.4 mm(2)) on the restored teeth (area 1; tooth area 1.0mm away from the border of resin composite restoration: area 2; tooth border area 0.3mm away from margin of resin composite restoration: area 3; resin composite border area 0.3mm away from margin of resin composite restoration: area 4; resin composite area at the center of resin composite restoration) using a spectrophotometer (Crystaleye). The color of each area was determined according to the CIELAB color scale. Color differences (ΔE*) between the areas of 1 and 2, 2 and 3, 3 and 4 and 1 and 4 were calculated, and also the ratio of ΔE*area2-3 to ΔE*area1-4 (ΔE*area2-3/1-4), ΔE*area3-4 to ΔE*area1-4 (ΔE*area3-4/1-4) and ΔE*area1-2 to ΔE*area1-4 (ΔE*area1-2/1-4) as a parameter of the color shift in resin composite restoration, were determined. Moreover, the light transmission characteristics of the resin materials and dentine discs from the younger and older teeth were measured using a goniophotometer. The data were statistically analyzed using two-way ANOVA, and Dunnett’s T3 and t-test for the post-hoc test.
RESULTS: ΔE*area2-3 (color difference between resin composite and tooth at the border) and ΔE*area1-4 (color difference between resin composite and tooth) of the older teeth groups were significantly larger than those of younger teeth groups (p<0.05). The ΔE*area2-3/1-4 (mis-match rate in color shifting at the border) of the older teeth groups was larger than that of the younger teeth groups (p<0.05). ΔE*area3-4/1-4 (color shifting rate of resin composite side) was significantly larger in older teeth than younger teeth (p<0.05), while ΔE*area1-2/1-4 (color shifting rate of tooth side), was significantly smaller in older teeth than younger teeth (p<0.05). In each tooth group, there were no significant differences in ΔE*area2-3, ΔE*area1-4, ΔE*area2-3/1-4, ΔE*area3-4/1-4 and ΔE*area1-2/1-4 between the materials (p>0.05). Analysis of the light transmission properties indicated that older dentine transmitted more light, while younger dentine exhibited greater light diffusion and transmitted less light.
CONCLUSIONS: The color shifting effects at the border of the resin composite restorations were influenced by the age of the tooth. This behavior might be influenced by the light transmission characteristics of dentine in restored teeth. Clinical Significance: The potential for color adjustment of resin composite restorations may be less in older teeth than younger teeth.
( 25242100)- as supplied by publisher]
- Effect of hesperidin incorporation into a self-etching primer on durability of dentin bond.
Effect of hesperidin incorporation into a self-etching primer on durability of dentin bond.
Dent Mater. 2014 Sep 3;
Authors: Islam MS, Hiraishi N, Nassar M, Yiu C, Otsuki M, Tagami J
OBJECTIVE: Collagen degradation at the resin-dentin interface deteriorates dentin bond durability. The use of natural cross-linkers might offer a positive approach to stabilize the resin-dentin interface. This study evaluated the effects of incorporation of natural cross-linkers into a self-etch adhesive primer on the immediate and long-term micro-tensile bond strengths (μTBS) to dentin.
METHODS: Experimental primers were prepared by incorporating either 0.5%, 1%, 2%, 5% of hesperidin (HPN) or 0.5% of proanthocyanidins (PA) into Clearfil SE primer. Extracted human molar teeth were restored using the experimental primers or the pure SE primer (control). The mechanical properties of the bonded interfaces were measured using the nano-indentation tests. Beam-shaped bonded specimens were sub-divided for one-day and one-year μTBS test. Interfacial collagen morphology was observed using transmission electron microscopy.
RESULT: The immediate μTBS significantly increased in 0.5%, 1% and 2% HPN-incorporated groups when compared with the control. The mechanical properties of bonded interface were improved with 1% and 2% HPN-incorporated primers. For the long-term μTBS, the 2% and 5% HPN-incorporated group were significantly higher than the control. The morphology of the collagen fibrils were preserved by 5% HPN-incorporation after one-year storage. The PA group, however, failed to improve the μTBS and the mechanical properties of the bonded interfaces.
SIGNIFICANCE: The incorporation of 2% HPN into the self-etching primer had a positive effect on the immediate μTBS and mechanical properties of the resin-dentin interfaces. The 5% HPN group preserved the morphology of the collagen in the hybrid layer after one-year storage in artificial saliva.
( 25194169)- as supplied by publisher]
- Effects of light curing method and resin composite composition on composite adaptation to the cavity wall.
Effects of light curing method and resin composite composition on composite adaptation to the cavity wall.
Dent Mater J. 2014 Jul 2;
Authors: Yoshikawa T, Morigami M, Sadr A, Tagami J
This study aimed to evaluate the effects of the light curing method and resin composite composition on marginal sealing and resin composite adaptation to the cavity wall. Cylindrical cavities were prepared on the buccal or lingual cervical regions. The teeth were restored using Clearfil Liner Bond 2V adhesive system and filled with Clearfil Photo Bright or Palfique Estelite resin composite. The resins were cured using the conventional or slow-start light curing method. After thermal cycling, the specimens were subjected to a dye penetration test. The slow-start curing method showed better resin composite adaptation to the cavity wall for both composites. Furthermore, the slow-start curing method resulted in significantly improved dentin marginal sealing compared with the conventional method for Clearfil Photo Bright. The light-cured resin composite, which exhibited increased contrast ratios duringpolymerization, seems to suggest high compensation for polymerization contraction stress when using the slow-start curing method.
( 24988883)- as supplied by publisher]
- Sealing performance of resin cements before and after thermal cycling: Evaluation by optical coherence tomography.
Sealing performance of resin cements before and after thermal cycling: Evaluation by optical coherence tomography.
Dent Mater. 2014 Jun 16;
Authors: Turkistani A, Sadr A, Shimada Y, Nikaido T, Sumi Y, Tagami J
OBJECTIVES: Self-adhesive resin cements have been recently introduced; however, there is little data available on their long-term performance. In this in vitro study, swept-source optical coherence tomography (OCT) at 1310nm center wavelength was used for monitoring adaptation of indirect resin restorations after thermal cycling.
METHODS: Resin inlays were luted to class-I cavities of extracted human teeth using three resin cements; Clearfil SA Luting (SA; Kuraray), Bistite II DC or Multibond II (Tokuyama Dental). Each cement was applied with or without pre-coating of dentin by a self-etch adhesive (Clearfil SE Bond) and a low-viscosity microfilled resin. OCT imaging was performed after 24h, after 2000 and after 10,000 thermocycles (n=5). Selected samples were sectioned for interfacial observation by confocal laser scanning microscope (CLSM). Floor adaptation (percentage) was analyzed by software on 20 B-scans throughout each specimen, and subjected to statistical analysis by three-way ANOVA test at a significance level of 0.05.
RESULTS: Resin cement type, resin coating and thermal aging all significantly affected adaptation (p<0.05). Initially, SA showed the highest adaptation; however, thermal aging significantly affected its sealing. The best results for all the cements were consistently achieved when the resin coating technique was applied where no deterioration of interfacial integrity was observed in the coated groups. CLSM closely confirmed OCT findings in all groups.
SIGNIFICANCE: OCT could be used for monitoring of composite inlays with several interfacial resin layers. The application of a direct bonding agent in the resin-coating technique improved interfacial sealing and durability of all resin cements.
( 24946983)- as supplied by publisher]
- Evaluation of the marginal fit at implant-abutment interface by optical coherence tomography.
Evaluation of the marginal fit at implant-abutment interface by optical coherence tomography.
J Biomed Opt. 2014 May 1;19(5):55002
Authors: Kikuchi K, Akiba N, Sadr A, Sumi Y, Tagami J, Minakuchi S
ABSTRACT. Vertical misfit of implant-abutment interface can affect the success of implant treatment; however, currently available modalities have limitations to detect these gaps. This study aimed to evaluate implant-abutment gaps in vitro using optical coherence tomography (OCT). Vertical misfit gaps sized 50, 100, 150, or 200 μm were created between external hexagonal implants and titanium abutments (Nobel Biocare, Göteborg, Sweden). A porcine gingival tissue slice, 0.5, 1.0, 1.5, or 2.0 mm in thickness, was placed on each implant-abutment interface. The gaps were evaluated by swept-source OCT at a center wavelength of 1330 nm (Panasonic Healthcare, Ehime, Japan) with beam angles of 90, 75 and 60 deg to the implant long-axis. The results suggested that while the measurements were precise, gap size and gingival thickness affected the sensitivity of detection. Gaps sized 100 μm and above could be detected with good accuracy under 0.5- or 1.0-mm-thick gingiva (GN). Around 70% of gaps sized 150 μm and above could be detected under 1.5-mm-thick GN. On the other hand, 80% of gaps under 2.0-mm-thick GN were not detected due to attenuation of near-infrared light through the soft tissue. OCT appeared as an effective tool for evaluating the misfit of implant-abutment under thin layers of soft tissue.
( 24805806)- in process]
- Endodontic instruments after torsional failure: Nanoindentation test.
Endodontic instruments after torsional failure: Nanoindentation test.
Scanning. 2014 Mar 9;
Authors: Jamleh A, Sadr A, Nomura N, Ebihara A, Yahata Y, Hanawa T, Tagami J, Suda H
This study aimed to evaluate effects of torsional loading on the mechanical properties of endodontic instruments using the nanoindentation technique. ProFile (PF; size 30, taper 04; Dentsply Maillefer, Switzerland) and stainless steel (SS; size 30, taper 02; Mani, Japan) instruments were subjected to torsional test. Nanoindentation was then performed adjacent to the edge of fracture (edge) and at the cutting part beside the shank (shank). Hardness and elastic modulus were measured under 100-mN force on 100 locations at each region, and compared to those obtained from the same regions on new instruments. It showed that PF and SS instruments failed at 559 ± 67 and 596 ± 73 rotation degrees and mean maximum torque of 0.90 ± 0.07 and 0.99 ± 0.05 N-cm, respectively. Hardness and elastic modulus ranged 4.8-6.7 and 118-339 GPa in SS, and 2.7-3.2 and 52-81 GPa in PF. Significant differences between torsion-fractured and new instruments in hardness and elastic modulus were detected in the SS system used. While in PF system, the edge region after torsional fracture had significantly lower hardness and elastic modulus compared to new instruments. The local hardness and modulus of elasticity of endodontic instruments adjacent to the fracture edge are significantly reduced by torsional loading. SCANNING 9999:1-7, 2014. © 2014 Wiley Periodicals, Inc.
( 24610598)- as supplied by publisher]
- Characterization of transparent dentin in attrited teeth using optical coherence tomography.
Related Articles on PubMed
Characterization of transparent dentin in attrited teeth using optical coherence tomography.
Lasers Med Sci. 2014 Feb 16;
Authors: Mandurah MM, Sadr A, Bakhsh TA, Shimada Y, Sumi Y, Tagami J
Attrition and wear of tooth surface occur with aging and result in loss of enamel, with exposure and histological changes in dentin. Dealing with attrited teeth and restoration of the lost tissue are clinically challenging. The main objective of this study is to characterize the exposed transparent dentin in the occlusal surface of attrited teeth by optical coherence tomography (OCT). Naturally attrited, extracted human teeth with occlusal-transparent dentin were investigated in comparison to sound and carious teeth. The teeth were subjected to OCT imaging and then cross-sectioned and polished. OCT B-scans were compared to light microscopy images of the same cross section. In OCT images, some changes were evident at the transparent dentin in attrited teeth. An OCT attenuation coefficient parameter (μ t) was derived based on the Beer-Lambert law as a function of backscatter signal slope. The mean values of μ t were 1.05 ± 0.3, 2.23 ± 0.4, and 0.61 ± 0.27 mm(-1) for sound, carious, and transparent dentins, respectively. One-way ANOVA with Tukey’s post-hoc showed a significant difference between groups (p < 0.05). Physiological changes in transparent dentin that involve deposition of mineral casts in the dentinal tubules lead to lower attenuation of OCT signal. OCT has a potential role to detect transparent dentin on the surface of attrited teeth and can be used in the future as a clinical adjunct tool.
PMID: 24532117 [PubMed - as supplied by publisher]
- Detection of occlusal caries in primary teeth using swept source optical coherence tomography.
Related Articles on PubMed
Detection of occlusal caries in primary teeth using swept source optical coherence tomography.
J Biomed Opt. 2014 Jan 1;19(1):16020
Authors: Nakajima Y, Shimada Y, Sadr A, Wada I, Miyashin M, Takagi Y, Tagami J, Sumi Y
ABSTRACT. This study aimed to investigate swept source optical coherence tomography (SS-OCT) as a detecting tool for occlusal caries in primary teeth. At the in vitro part of the study, 38 investigation sites of occlusal fissures (noncavitated and cavitated) were selected from 26 extracted primary teeth and inspected visually using conventional dental equipment by six examiners without any magnification. SS-OCT cross-sectional images at 1330-nm center wavelength were acquired on the same locations. The teeth were then sectioned at the investigation site and directly viewed under a confocal laser scanning microscope (CLSM) by two experienced examiners. The presence and extent of caries were scored in each observation. The results obtained from SS-OCT and conventional visual inspections were compared with those of CLSM. Consequently, SS-OCT could successfully detect both cavitated and noncavitated lesions. The magnitude of sensitivity for SS-OCT was higher than those for visual inspection (sensitivity of visual inspection and SS-OCT, 0.70 versus 0.93 for enamel demineralization, 0.49 versus 0.89 for enamel cavitated caries, and 0.36 versus 0.75 for dentin caries). Additionally, occlusal caries of a few clinical cases were observed using SS-OCT in vivo. The results indicate that SS-OCT has a great detecting potential for occlusal caries in primary teeth.
PMID: 24474506 [PubMed - in process]
- Evaluation of new treatment for incipient enamel demineralization using 45S5 bioglass.
Related Articles on PubMed
Evaluation of new treatment for incipient enamel demineralization using 45S5 bioglass.
Dent Mater. 2014 Jan 13;
Authors: Bakry AS, Takahashi H, Otsuki M, Tagami J
Bioglass 45S5 is a silica-based bioactive glass capable of depositing a layer of hydroxyl carbonate apatite on the surface of the glass when immersed in body fluids. The present paper studies a new technique for treating early human dental enamel caries lesions by using a paste composed of 45S5 bioglass and phosphoric acid. Artificial caries lesions were induced in enamel flat surfaces by means of a decalcification solution. All specimens were exposed to a brushing-abrasion challenge to test the durability of any newly formed layer resulting from the application of 45S5 bioglass paste. The specimens treated with bioglass paste showed complete coverage with a layer of brushite crystals. The brushing-abrasion challenge did not statistically affect the percentage of enamel coverage with the crystalline layer formed by the application of bioglass (p<0.05). These crystals were converted to hydroxyapatite crystals when stored in artificial saliva for 14 days. The current technique suggests the possibility of restoring incipient enamel erosive lesion with an abrasion durable layer of hydroxyapatite crystals.
PMID: 24433821 [PubMed - as supplied by publisher]
- Nanoindentation hardness of intertubular dentin in sound, demineralized and natural caries-affected dentin.
Related Articles on PubMed
Nanoindentation hardness of intertubular dentin in sound, demineralized and natural caries-affected dentin.
J Mech Behav Biomed Mater. 2013 Dec 24;32C:39-45
Authors: Joves GJ, Inoue G, Sadr A, Nikaido T, Tagami J
The purpose of this study was to investigate the mechanical properties of intertubular dentin in sound, natural caries-affected (NCAD) and artificial caries-affected dentin (ACAD) using nanoindentation.
MATERIALS AND METHODS: Non-caries molars and caries molars with International Caries Detection and Assessment System (ICDAS II) score 5 at the occlusal site were used and caries was excavated using a spoon excavator, a round bur at low speed without water and a dye solution as guidance to detect the infected tissue. Specimens with remaining dentin thickness (RDT) >2mm were selected. ACAD teeth were created from sound teeth over 7 days in a demineralizing solution. Specimens were embedded into plastic rings with acrylic resin and then sagittal mesial-distal sectioned from crown to the long axis of the root under cooling water using a low-speed diamond blade. The surface of interest was fine polished sequentially. Hardness measurement was performed within an axial depth of 1000μm with at least of 320 indentations on each sample. Mann-Whitney U Test was used to compare the hardness as the variable among different dentin types (SOUND, NCAD and ACAD) at each dentin depth level.
RESULTS: There was no significant difference in nanohardness between NCAD and ACAD up to a depth of 130μm (p>0.05). NCAD consistently showed lower hardness. ACAD showed no significant difference in hardness with SOUND dentin beyond 190μm (p<0.05). The lesion front in ACAD was considered to be located around the depth of 180μm.
CONCLUSION: Natural and artificial caries-affected dentin tissues were superficially comparable in intertubular nanohardness. There is a certain layer within the natural caries-affected dentin with higher hardness; however the long-term effects of caries beneath the lesion extend deeply through intertubular dentin. Sound dentin at deep areas (close to the pulp chamber) is considered to be soft.
PMID: 24394774 [PubMed - as supplied by publisher]
- Dental Pulp Dendritic Cells Migrate to Regional Lymph Nodes.
Related Articles on PubMed
Dental Pulp Dendritic Cells Migrate to Regional Lymph Nodes.
J Dent Res. 2013 Dec 30;
Authors: Bhingare AC, Ohno T, Tomura M, Zhang C, Aramaki O, Otsuki M, Tagami J, Azuma M
Dendritic cell (DC) migration to regional lymph nodes (RLNs) is an essential step in adaptive immunity, and cell-surface antigens on migrating DCs greatly affect the quality and quantity of subsequent immune responses. Although MHC class II(+) DC-like cells exist in the dental pulp, the lineage and function of these cells remain unknown. Here, we identified migratory DCs from the dental pulp after cusp trimming and acid etching in KikGR mice, in which the photoconvertible fluorescent protein changed from green to red upon violet light exposure. Two major cell fractions from the dental pulp had migrated to the RLNs at 16 hrs after cusp treatment, which showed the following lineage markers in the main and second fractions: CD11c(high)CD11b(++)Ly6C(low) Ly6G(low) F4/80(+) and CD11c(med)CD11b(+++)Ly6C(++)Ly6G(+++)F4/80(-), respectively. These lineage markers indicate that the former cells were DCs that had migrated through afferent lymphoid vessels, and the latter were granulocytes recruited via blood circulation. Migratory dental pulp DCs were mature, expressing the highest levels of CD273 (B7-DC) and CD86 co-stimulators and MHC class II. Our results suggest that cariogenic-bacteria-exposed dental pulp DCs migrate to RLNs and there trigger adaptive immune responses.
PMID: 24378366 [PubMed - as supplied by publisher]
- Effect of smear layer deproteinizing on resin-dentine interface with self-etch adhesive.
Related Articles on PubMed
Effect of smear layer deproteinizing on resin-dentine interface with self-etch adhesive.
J Dent. 2013 Dec 7;
Authors: Thanatvarakorn O, Nakajima M, Prasansuttiporn T, Ichinose S, Foxton RM, Tagami J
OBJECTIVES: This study aimed to investigate deproteinizing effect of sodium-hypochlorite (NaOCl) and mild acidic hypochlorous-acid (HOCl) pretreatment on smear layer-covered dentine and to evaluate their effects on morphological characteristics of resin-dentine interface with self-etch adhesive.
METHODS: Human coronal-dentine discs with standardized smear layer were pretreated with 6% NaOCl or 50ppm HOCl for 15s or 30s. Their deproteinizing effects at the treated smear layer-covered dentine surfaces were determined by the measurement of amide:phosphate ratio using ATR-FTIR analysis. In addition, using TEM, micromorphological alterations of hybridized complex and nanoleakage expression were evaluated at the interface of a self-etch adhesive (Clearfil SE Bond) to the pretreated dentine surface with or without subsequent application of a reducing agent (p-toluenesulfinic acid salt; Accel(®)).
RESULTS: Both pretreatments of NaOCl and HOCl significantly reduced the amide:phosphate ratio as compared with the no-pretreated group (p<0.05), coincident with the elimination of the hybridized smear layer on their bonded interfaces. Nanoleakage within the hybrid layer was found in the no-pretreated and NaOCl-pretreated groups, whereas the subsequent reducing agent application changed the reticular nanoleakage to spotted type. HOCl-pretreated groups showed less nanoleakage expression in a spotted pattern, regardless of reducing agent application.
CONCLUSIONS: NaOCl and HOCl solutions could remove the organic component on the smear layer-covered dentine, which could eliminate the hybridized smear layer created by self-etch adhesive, leading to the reduction of nanoleakage expression within hybrid layer.
CLINICAL SIGNIFICANCE: Smear layer deproteinizing could modify dentine surface, giving an appropriate substrate for bonding to self-etch adhesive system.
PMID: 24321293 [PubMed - as supplied by publisher]
- The inhibition effect of non-protein thiols on dentinal matrix metalloproteinase activity and HEMA cytotoxicity.
Related Articles on PubMed
The inhibition effect of non-protein thiols on dentinal matrix metalloproteinase activity and HEMA cytotoxicity.
J Dent. 2013 Dec 4;
Authors: Nassar M, Hiraishi N, Shimokawa H, Tamura Y, Otsuki M, Kasugai S, Ohya K, Tagami J
OBJECTIVES: Phosphoric acid (PA) etching used in etch-and-rinse adhesives is known to activate host-derived dentinal matrix-metalloproteinases (MMPs) and increase dentinal permeability. These two phenomena will result, respectively; in degradation of dentin-adhesive bond and leaching of some monomers especially 2-hydroxyethyl methacrylate (HEMA) into the pulp that would negatively affect the viability of pulpal cells. This study is the first to investigate the inhibitory effect of non-protein thiols (NPSH); namely reduced glutathione (GSH) and N-acetylcysteine (NAC) on dentinal MMPs and compare their effects on HEMA cytotoxicity.
METHODS: Dentin powder was prepared from human teeth, demineralized with 1% PA and then treated with 2% GSH, 2% NAC or 2% chlorhexidine (CHX). Zymographic analysis of extracted proteins was performed. To evaluate the effect of GSH, NAC and CHX on HEMA cytotoxicity, solutions of these compounds were prepared with or without HEMA and rat pulpal cells were treated with the tested solutions for (6 and 24h). Cells viability was measured by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cytotoxicity data were analyzed by one-way ANOVA and Tukey post-hoc tests (P<0.05). Results: The inhibitory effect of GSH and NAC on dentinal MMPs was confirmed. GSH showed similar effectiveness to NAC regarding HEMA cytotoxicity inhibition.
CONCLUSION: NPSH were effective to inhibit dentinal MMPs and HEMA cytotoxicity.
CLINICAL SIGNIFICANCE: The tested properties of NPSH provide promising clinical use of these agents which would enhance dentin-bond durability and decrease post-operative sensitivity.
PMID: 24316344 [PubMed - as supplied by publisher]
- Acceleration of curing of resin composite at the bottom surface using slow-start curing methods.
Acceleration of curing of resin composite at the bottom surface using slow-start curing methods.
Dent Mater J. 2013 Nov 15;
Authors: Yoshikawa T, Morigami M, Sadr A, Tagami J
The aim of this study was to evaluate the effect of two slow-start curing methods on acceleration of the curing of resin composite specimens at the bottom surface. The light-cured resin composite was polymerized using one of three curing techniques: (1) 600 mW/cm(2) for 60 s, (2) 270 mW/cm(2) for 10 s+0-s interval+600 mW/cm(2) for 50 s, and (3) 270 mW/cm(2) for 10 s+5-s interval+600 mW/cm(2) for 50 s. After light curing, Knoop hardness number was measured at the top and bottom surfaces of the resin specimens. The slow-start curing method with the 5-s interval caused greater acceleration of curing of the resin composite at the bottom surface of the specimens than the slow-start curing method with the 0-s interval. The light-cured resin composite, which had increased contrast ratios during polymerization, showed acceleration of curing at the bottom surface.
PMID: 24240907 [PubMed - as supplied by publisher]
- Polymerization behavior within adhesive layer of one- and two-step self-etch adhesives: A micro-Raman spectroscopic study.
Polymerization behavior within adhesive layer of one- and two-step self-etch adhesives: A micro-Raman spectroscopic study.
Dent Mater J. 2013 Nov 15;
Authors: Sakano W, Nakajima M, Prasansuttiporn T, M Foxton R, Tagami J
This study investigated the polymerization behavior within the adhesive layer of one- and two-step self-etch adhesives at the dentincomposite interface. Dentin surfaces were applied with Clearfil S(3) Bond (TS), Clearfil S(3) Bond Plus (TSP) and Clearfil SE Bond (SE), and then placed with a light-curing resin composite. After water storage for 24 h, the bonded teeth were sectioned and polished perpendicular to the adhesive interface, and the degree of conversion (DC) of the adhesive layer between the dentin and composite were determined using micro-Raman analysis. For all the adhesives, the DCs of the adhesive layers significantly decreased near the adhesive-composite join (p<0.05). For the maximum DC value (Pmax) and the DC value at the adhesive-composite join (Pitf), TS was significantly lower than TSP and SE (p<0.05). The polymerization of oxygen-inhibited layer at the top of the adhesive could not reach maximum DC even after polymerization of the overlying resin composite.
PMID: 24240894 [PubMed - as supplied by publisher]
- The effect of glutathione on 2-hydroxyethylmethacrylate cytotoxicity and on resin-dentine bond strength.
Related Articles on PubMed
The effect of glutathione on 2-hydroxyethylmethacrylate cytotoxicity and on resin-dentine bond strength.
Int Endod J. 2013 Oct 9;
To evaluate the influence of reduced glutathione (GSH) application on 2-hydroxyethylmethacrylate (HEMA) cytotoxicity on rat pulpal cells and evaluate the effect of etched-dentine treatment with GSH on the immediate microtensile bond strength (μTBS) of etch-and-rinse adhesive.
The cytotoxicity of 10 mmol L-1 HEMA, 10 mmol L-1 HEMA + 1 mmol L-1 GSH, 10 mmol L-1 HEMA + 5 mmol L-1 GSH and 10 mmol L-1 HEMA + 10 mmol L-1 GSH was compared (6 h and 24 h). Cells viability was measured by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, followed by morphological observation of cells. Etched-dentine surfaces were rinsed and treated with one of the following solutions: 2% GSH, 5% GSH or 10% GSH, bonded with Adper Single Bond Plus (3M, ESPE, St. Paul, MN, USA) and restored with resin composite. The control group received no GSH treatment. After 1 day of water-storage at 37 °C, the specimens were subjected to μTBS testing. Cytotoxicity and μTBS data were analysed by one-way anova and Tukey post hoc tests (P < 0.05).
There were significant differences between the groups. HEMA elicited a remarkable toxic effect. 10 mmol L-1 GSH prevented HEMA-induced damage at both exposure times. Whilst 5 mmol L-1 GSH lost its protective effect at 24-h exposure time and 1 mmol L-1 GSH showed no protective effect at both exposure times, GSH had no significant effect on the immediate μTBS; however, 5% GSH had higher bond strength value when compared to 10% GSH (P = 0.003).
Controlled concentrations of GSH had a protective effect against HEMA cytotoxicity. GSH had neither positive nor negative influence on μTBS.
PMID: 24117849 [PubMed - as supplied by publisher]
- Effects of solvent drying time on mass change of three adhesives.
Related Articles on PubMed
Effects of solvent drying time on mass change of three adhesives.
J Conserv Dent. 2013 9;16(5):418-422
AIM: Adhesives may change their mass due to water sorption or dilution of components after curing. The aim of this study was to evaluate the influence of air-drying time and water storage on mass changes (MC) of three adhesives; Adper Single Bond2 (ASB), One-step plus (OSP), Clearfil S(3) Bond (CSB).
MATERIALS AND METHODS: Rectangular-shape samples from each adhesive were prepared and cured for 120 s with a halogen light curing unit. Prior to curing, their solvent was evaporated by means of three different procedures depending on the passive air-drying time (i.e., no air drying, equal to active air drying, complete evaporation after 3 h). Each group was further divided into two subgroups based on the time of water storage (1-day, 7-days), prior to measurement of MC (n = 10). The data were analyzed using a three-way ANOVA.
RESULTS: Adhesives showed different patterns of MC in relation to air drying and water storage; (P < 0.05). In OSP and CSB with increasing water storage and air drying, the MC increased significantly (P < 0.01).
CONCLUSION: The highest MC in the etch-and-rinse adhesives was observed when the adhesive was not dried, while in the self-etch adhesive the highest changes were observed when the adhesive was completely dried.
PMID: 24082570 [PubMed - as supplied by publisher]
- In vitro evaluation of plant-derived agents to preserve dentin collagen.
In vitro evaluation of plant-derived agents to preserve dentin collagen.
Dent Mater. 2013 Aug 10;
Authors: Hiraishi N, Sono R, Sofiqul I, Yiu C, Nakamura H, Otsuki M, Takatsuka T, Tagami J
OBJECTIVE: Biomodification of dentin by a natural crosslinker has been recommended to improve a mechanical property of demineralized dentin. This study investigated the effect of various plant-derived agents (hesperidin, proanthocyanidin, epigallocatechin gallate and genipin) on the stability of dentin collagen matrix to resist collagenase degradation.
METHODS: The dentin specimens were treated with glutaraldehyde (0.5% and 5.0%) and each plant-derived test solution (0.5%). They were subjected to ultimate tensile strength (UTS) and swelling ratio measurements. Demineralized human dentin powder was incubated with 0.02%, 0.1% and 0.5% of each test agent and followed by bacterial collagenase digestion. The extent of collagen degradation was investigated using hydroxyproline assay.
RESULTS: The UTS and swelling ratio measurements revealed that the mechanical property of dentin was improved by the use of these natural agents. The greatest reduction in collagen degradation was shown following the use of hesperidin, proanthocyanidin, and epigallocatechin gallate at 0.5%.
SIGNIFICANCE: The use of hesperidin, proanthocyanidin, and epigallocatechin gallate could improve the mechanical properties of collagen and resist enzymatic degradation, leading to functional repair of pathological dentin lesion.
PMID: 23942145 [PubMed - as supplied by publisher]
- The importance of size-exclusion characteristics of type I collagen in bonding to dentin matrices.
The importance of size-exclusion characteristics of type I collagen in bonding to dentin matrices.
Acta Biomater. 2013 Aug 5;
Authors: Takahashi M, Nakajima M, Tagami J, Scheffel DL, Carvalho RM, Mazzoni A, Carrilho M, Tezvergil-Mutluay A, Breschi L, Tjäderhane L, Jang SS, Tay FR, Agee KA, Pashley DH
The mineral phase of dentin is located primarily within collagen fibrils. During development, bone or dentin collagen fibrils are formed first and then water within the fibril is replaced with apatite crystallites. Mineralized collagen contains very little water. During dentin bonding, acid-etching of mineralized dentin solubilizes the mineral crystallites and replaces them with water. During the infiltration phase of dentin bonding, adhesive comonomers are supposed to replace all of the collagen water with adhesive monomers that are then polymerized into copolymers. The authors of a recently published review suggested that dental monomers were too large to enter and displace water from collagen fibrils. If that were true, the endogenous proteases bound to dentin collagen could be responsible for unimpeded collagen degradation that is responsible for the poor durability of resin-dentin bonds. The current work studied the size-exclusion characteristics of dentin collagen, using a gel-filtration-like column chromatography technique, using dentin powder instead of Sephadex. The elution volumes of test molecules, including adhesive monomers, revealed that adhesive monomers smaller than about 1000 Da can freely diffuse into collagen water, while molecules of 10,000 Da begin to be excluded, and bovine serum albumin (66,000 Da) was fully excluded. These results validate the concept that dental monomers can permeate between collagen molecules during infiltration by etch-and-rinse adhesives.
PMID: 23928333 [PubMed - as supplied by publisher]
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