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  • Dentin bonding performance using Weibull statistics and evaluation of acid-base resistant zone formation of recently introduced adhesives.

    Dentin bonding performance using Weibull statistics and evaluation of acid-base resistant zone formation of recently introduced adhesives.

    Dent Mater J. 2016 Jun 23;

    Authors: Guan R, Takagaki T, Matsui N, Sato T, Burrow MF, Palamara J, Nikaido T, Tagami J

    Abstract
    Dentin bonding durability of recently introduced dental adhesives: Clearfil SE Bond 2 (SE2), Optibond XTR (XTR), and Scotchbond Universal (SBU) was investigated using Weibull analysis as well as analysis of the micromorphological features of the acid-base resistant zone (ABRZ) created for the adhesives. The bonding procedures of SBU were divided into three subgroups: self-etch (SBS), phosphoric acid (PA) etching on moist (SBM) or dry dentin (SBD). All groups were thermocycled for 0, 5,000 and 10,000 cycles followed by microtensile bond strength testing. Acid-base challenge was undertaken before SEM and TEM observations of the adhesive interface. The etch-and-rinse method with SBU (SBM and SBD) created inferior interfaces on the dentin surface which resulted in reduced bond durability. ABRZ formation was detected with the self-etch adhesive systems; SE2, XTR and SBS. In the PA etching protocols of SBM and SBD, a thick hybrid layer but no ABRZ was detected, which might affect dentin bond durability.

    (27335136) – as supplied by publisher]

    Bibliography

  • Dentin bonding performance using Weibull statistics and evaluation of acid-base resistant zone formation of recently introduced adhesives.

    Dentin bonding performance using Weibull statistics and evaluation of acid-base resistant zone formation of recently introduced adhesives.

    Dent Mater J. 2016 Jun 23;

    Authors: Guan R, Takagaki T, Matsui N, Sato T, Burrow MF, Palamara J, Nikaido T, Tagami J

    Abstract
    Dentin bonding durability of recently introduced dental adhesives: Clearfil SE Bond 2 (SE2), Optibond XTR (XTR), and Scotchbond Universal (SBU) was investigated using Weibull analysis as well as analysis of the micromorphological features of the acid-base resistant zone (ABRZ) created for the adhesives. The bonding procedures of SBU were divided into three subgroups: self-etch (SBS), phosphoric acid (PA) etching on moist (SBM) or dry dentin (SBD). All groups were thermocycled for 0, 5,000 and 10,000 cycles followed by microtensile bond strength testing. Acid-base challenge was undertaken before SEM and TEM observations of the adhesive interface. The etch-and-rinse method with SBU (SBM and SBD) created inferior interfaces on the dentin surface which resulted in reduced bond durability. ABRZ formation was detected with the self-etch adhesive systems; SE2, XTR and SBS. In the PA etching protocols of SBM and SBD, a thick hybrid layer but no ABRZ was detected, which might affect dentin bond durability.

    (27335136) – as supplied by publisher]

    Bibliography

  • Dentin bonding performance and interface observation of an MMA-based restorative material.

    Dentin bonding performance and interface observation of an MMA-based restorative material.

    Dent Mater J. 2016 Jun 23;

    Authors: Shinagawa J, Inoue G, Nikaido T, Ikeda M, Sadr A, Tagami J

    Abstract
    The purpose of this study was to evaluate bonding performance and dentin interface acid resistance using a 4-META/MMA-TBB based restorative material (BF) compared to a conventional 4-META/MMA-TBB resin cement (SB), and the effect of sodium fluoride (NaF) addition to the materials. Dentin surfaces were treated with 10% citric acid-3% ferric chloride (10-3) or 4-META containing self-etching primer (TP), followed by application of BF or SB polymer powders with or without NaF, to evaluate microtensile bond strength (µTBS) in six experimental groups; 10-3/SB, 10-3/BF, TP/SB, TP/BF, TP/SB/NaF and TP/BF/NaF. SEM observation of the resin-dentin interface was performed after acid-base challenge to evaluate interfacial dentin resistance to acid attack. TP/BF showed highest µTBS, while NaF polymers decreased µTBS. TP/BF showed funnel-shaped erosion at the interface, however, NaF polymers improved acid resistance of interface. In conclusion, BF demonstrated high µTBSs and low acid-resistance at the interface. NaF addition enhanced acid resistance but decreased µTBS.

    (27335135) – as supplied by publisher]

    Bibliography

  • Dentin bonding performance and interface observation of an MMA-based restorative material.

    Dentin bonding performance and interface observation of an MMA-based restorative material.

    Dent Mater J. 2016 Jun 23;

    Authors: Shinagawa J, Inoue G, Nikaido T, Ikeda M, Sadr A, Tagami J

    Abstract
    The purpose of this study was to evaluate bonding performance and dentin interface acid resistance using a 4-META/MMA-TBB based restorative material (BF) compared to a conventional 4-META/MMA-TBB resin cement (SB), and the effect of sodium fluoride (NaF) addition to the materials. Dentin surfaces were treated with 10% citric acid-3% ferric chloride (10-3) or 4-META containing self-etching primer (TP), followed by application of BF or SB polymer powders with or without NaF, to evaluate microtensile bond strength (µTBS) in six experimental groups; 10-3/SB, 10-3/BF, TP/SB, TP/BF, TP/SB/NaF and TP/BF/NaF. SEM observation of the resin-dentin interface was performed after acid-base challenge to evaluate interfacial dentin resistance to acid attack. TP/BF showed highest µTBS, while NaF polymers decreased µTBS. TP/BF showed funnel-shaped erosion at the interface, however, NaF polymers improved acid resistance of interface. In conclusion, BF demonstrated high µTBSs and low acid-resistance at the interface. NaF addition enhanced acid resistance but decreased µTBS.

    (27335135) – as supplied by publisher]

    Bibliography

  • Influence of water immersion on the mechanical properties of fiber posts.
    .

    Influence of water immersion on the mechanical properties of fiber posts.

    J Prosthodont Res. 2016 Jun 17;

    Authors: Komada W, Inagaki T, Ueda Y, Omori S, Hosaka K, Tagami J, Miura H

    Abstract
    PURPOSE: The purpose of this study was to evaluate the influence of water immersion on the mechanical properties of three kinds of glass fiber posts and the fracture resistance of structures using resin composites with glass fiber posts.
    METHODS: Each post was divided into three groups; a control group and two water immersion groups (30 and 90 days). Flexural strength was determined by three-point bending test. Each structure was divided into two groups; a control group and a water immersion group for 30 days. The fracture strength of structures was determined by a static loading test.
    RESULTS: In the flexural strength, two kinds of post in water immersion groups showed lower values than control groups. In the fracture strength, two kinds of structures in water immersion group showed lower values than control groups.
    CONCLUSION: The prefabricated glass fiber posts and structures using resin composites with glass fiber posts were affected by water immersion.

    (27324293) – as supplied by publisher]

    Bibliography

  • Influence of water immersion on the mechanical properties of fiber posts.
    .

    Influence of water immersion on the mechanical properties of fiber posts.

    J Prosthodont Res. 2016 Jun 17;

    Authors: Komada W, Inagaki T, Ueda Y, Omori S, Hosaka K, Tagami J, Miura H

    Abstract
    PURPOSE: The purpose of this study was to evaluate the influence of water immersion on the mechanical properties of three kinds of glass fiber posts and the fracture resistance of structures using resin composites with glass fiber posts.
    METHODS: Each post was divided into three groups; a control group and two water immersion groups (30 and 90 days). Flexural strength was determined by three-point bending test. Each structure was divided into two groups; a control group and a water immersion group for 30 days. The fracture strength of structures was determined by a static loading test.
    RESULTS: In the flexural strength, two kinds of post in water immersion groups showed lower values than control groups. In the fracture strength, two kinds of structures in water immersion group showed lower values than control groups.
    CONCLUSION: The prefabricated glass fiber posts and structures using resin composites with glass fiber posts were affected by water immersion.

    (27324293) – as supplied by publisher]

    Bibliography

  • Effect of thermal cyclic stress on acid resistance of resin-infiltrated incipient enamel lesions in vitro.

    Effect of thermal cyclic stress on acid resistance of resin-infiltrated incipient enamel lesions in vitro.

    Dent Mater J. 2016;35(3):425-31

    Authors: Takashino N, Nakashima S, Shimada Y, Tagami J, Sumi Y

    Abstract
    This study aimed to examine the effect of thermal cycling on gap formation at the interface between infiltrated resin (ICON(®)) and enamel lesion and on the durability of anti-demineralization efficacy to predict the future performance. SS-OCT technique was examined to determine whether it has the potential to detect the gap. Bovine enamel lesions were prepared, and the infiltrated resin was applied to the lesion. Resin-infiltrated lesion specimens were thermal cycled 10,000 cycles and further demineralized in pH 4.5 buffer for 7 days. Released Ca (mg/cm(2)) was quantified by Ca electrode. The SS-OCT technique was applied to detect the gap, and SEM observation was performed to determine the presence of the gap. There was no significant difference in the amount of Ca release before and after the thermal cycling, suggesting long-lasting anti-demineralization efficacy of the resin. SS-OCT and SEM observations indicated no apparent gap formation after the thermal cycling.

    (27251998) – in process]

    Bibliography

  • Assessment of occlusal fissure depth and sealant penetration using optical coherence tomography.

    Assessment of occlusal fissure depth and sealant penetration using optical coherence tomography.

    Dent Mater J. 2016;35(3):432-9

    Authors: Ito S, Shimada Y, Sadr A, Nakajima Y, Miyashin M, Tagami J, Sumi Y

    Abstract
    Swept source optical coherence tomography (SS-OCT) is an interferometric imaging technique. This study aimed to assess SS-OCT as a diagnostic tool for the evaluation of occlusal fissure depth and sealant penetration into fissure. Seventy investigation sites of occlusal fissures without visible evidence of caries were categorized into four groups (smooth, shallow, intermediate, and deep fissures). Categorization utilized two methods: 1) visually, using a computer screen, and 2) using SS-OCT images. After sealant placement, penetration was observed in SS-OCT. The results obtained from SS-OCT and visual inspections were compared with those of confocal laser scanning microscope (CLSM). The diagnostic power of SS-OCT was higher than that of visual inspection for fissure depth. Additionally, clear cross-sectional images of sealant penetration into fissures were observed with SS-OCT. SS-OCT can be used to evaluate fissure depth and monitor sealant penetration.

    (27251999) – in process]

    Bibliography

  • Assessment of occlusal fissure depth and sealant penetration using optical coherence tomography.

    Assessment of occlusal fissure depth and sealant penetration using optical coherence tomography.

    Dent Mater J. 2016;35(3):432-9

    Authors: Ito S, Shimada Y, Sadr A, Nakajima Y, Miyashin M, Tagami J, Sumi Y

    Abstract
    Swept source optical coherence tomography (SS-OCT) is an interferometric imaging technique. This study aimed to assess SS-OCT as a diagnostic tool for the evaluation of occlusal fissure depth and sealant penetration into fissure. Seventy investigation sites of occlusal fissures without visible evidence of caries were categorized into four groups (smooth, shallow, intermediate, and deep fissures). Categorization utilized two methods: 1) visually, using a computer screen, and 2) using SS-OCT images. After sealant placement, penetration was observed in SS-OCT. The results obtained from SS-OCT and visual inspections were compared with those of confocal laser scanning microscope (CLSM). The diagnostic power of SS-OCT was higher than that of visual inspection for fissure depth. Additionally, clear cross-sectional images of sealant penetration into fissures were observed with SS-OCT. SS-OCT can be used to evaluate fissure depth and monitor sealant penetration.

    (27251999) – in process]

    Bibliography

  • Effect of thermal cyclic stress on acid resistance of resin-infiltrated incipient enamel lesions in vitro.

    Effect of thermal cyclic stress on acid resistance of resin-infiltrated incipient enamel lesions in vitro.

    Dent Mater J. 2016;35(3):425-31

    Authors: Takashino N, Nakashima S, Shimada Y, Tagami J, Sumi Y

    Abstract
    This study aimed to examine the effect of thermal cycling on gap formation at the interface between infiltrated resin (ICON(®)) and enamel lesion and on the durability of anti-demineralization efficacy to predict the future performance. SS-OCT technique was examined to determine whether it has the potential to detect the gap. Bovine enamel lesions were prepared, and the infiltrated resin was applied to the lesion. Resin-infiltrated lesion specimens were thermal cycled 10,000 cycles and further demineralized in pH 4.5 buffer for 7 days. Released Ca (mg/cm(2)) was quantified by Ca electrode. The SS-OCT technique was applied to detect the gap, and SEM observation was performed to determine the presence of the gap. There was no significant difference in the amount of Ca release before and after the thermal cycling, suggesting long-lasting anti-demineralization efficacy of the resin. SS-OCT and SEM observations indicated no apparent gap formation after the thermal cycling.

    (27251998) – in process]

    Bibliography

  • Morphological Evaluation of the Adhesive/Enamel interfaces of Two-step Self-etching Adhesives and Multimode One-bottle Self-etching Adhesives.
    .

    Morphological Evaluation of the Adhesive/Enamel interfaces of Two-step Self-etching Adhesives and Multimode One-bottle Self-etching Adhesives.

    J Adhes Dent. 2016 May 19;

    Authors: Sato T, Takagaki T, Matsui N, Hamba H, Sadr A, Nikaido T, Tagami J

    Abstract
    PURPOSE: To evaluate the acid-base resistant zone (ABRZ) at the adhesive/enamel interface of self-etching adhesives with or without prior phosphoric acid etching.
    MATERIALS AND METHODS: Four adhesives were used in 8 groups: Clearfil SE Bond (SEB), Optibond XTR (XTR), Scotchbond Universal Adhesive (SBU), and Clearfil BOND SE ONE (ONE) without prior phosphoric-acid etching, and each adhesive with phosphoric acid etching for 10 s (P-SEB, P-XTR, P-SBU and P-ONE, respectively). After application of self-etching adhesives on ground enamel surfaces of human teeth, a flowable composite was placed. For observation of the acid-base resistant zone (ABRZ), the bonded interface was exposed to demineralizing solution (pH 4.5) for 4.5 h, followed by 5% NaOCl with ultrasonication for 20 min. After the acid-base challenge, morphological attributes of the interface were observed using SEM.
    RESULTS: ABRZ formation was confirmed in all groups. The funnel-shaped erosion beneath the interface was present in SBU and ONE, where nearly 10 to 15 μm of enamel was dissolved. With phosphoric acid etching, the ABRZs were obviously thicker compared with no phosphoric acid etching.
    CONCLUSION: Enamel beneath the bonding interface was more susceptible to acid dissolution in SBU and ONE. In the case of the one-bottle self-etching adhesives and universal adhesives that intrinsically have higher pH values, enamel etching should be recommended to improve the interfacial quality.

    (27200432) – as supplied by publisher]

    Bibliography

  • Effect of Scrubbing Technique with Mild Self-etching Adhesives on Dentin Bond Strengths and Nanoleakage Expression.

    Effect of Scrubbing Technique with Mild Self-etching Adhesives on Dentin Bond Strengths and Nanoleakage Expression.

    J Adhes Dent. 2016 May 9;

    Authors: Thanatvarakorn O, Prasansuttiporn T, Takahashi M, Thittaweerat S, Foxton RM, Ichinose S, Tagami J, Nakajima M

    Abstract
    PURPOSE: To evaluate the effect of a scrubbing technique with one-step self-etching adhesives on bond strengths and nanoleakage expression at the resin/dentin interface.
    MATERIALS AND METHODS: Flat human dentin surfaces bonded with one of two mild self-etching adhesives, SE One (SE) or Scotchbond Universal (SU) applied either with scrubbing or without scrubbing technique, were prepared (n = 5). The microtensile bond strengths (μTBS), SE micrographs of morphological changes on treated dentin surfaces, and expression of nanoleakage along the bonded dentin interfaces as shown with TEM were evaluated. μTBS data were analyzed using two-way ANOVA and the post-hoc t-test at the significance level of 0.05.
    RESULTS: The scrubbing technique had a significant positive effect on the μTBS of SU (p < 0.05), while it produced no significant difference for SE (p > 0.05). Morphological evaluation of the treated dentin surfaces demonstrated that SU with scrubbing showed the highest etching ability, followed by scrubbing SE > nonscrubbing SE > nonscrubbing SU. In the nonscrubbing groups, nanoleakage formation using SU exhibited a reticular pattern throughout the hybridized complex, whereas with SE, water-tree nanoleakage was only found in the adhesive layer at dentinal tubule orifices. The scrubbing groups of both adhesives did not exhibit any nanoleakage expression.
    CONCLUSION: Using a scrubbing technique when applying mild self-etching adhesives could improve resin monomer infiltration into dentin, chase water on adhesive surfaces, and facilitate smear layer removal.

    (27163111) – as supplied by publisher]

    Bibliography

  • Effect of Scrubbing Technique with Mild Self-etching Adhesives on Dentin Bond Strengths and Nanoleakage Expression.

    Effect of Scrubbing Technique with Mild Self-etching Adhesives on Dentin Bond Strengths and Nanoleakage Expression.

    J Adhes Dent. 2016 May 9;

    Authors: Thanatvarakorn O, Prasansuttiporn T, Takahashi M, Thittaweerat S, Foxton RM, Ichinose S, Tagami J, Nakajima M

    Abstract
    PURPOSE: To evaluate the effect of a scrubbing technique with one-step self-etching adhesives on bond strengths and nanoleakage expression at the resin/dentin interface.
    MATERIALS AND METHODS: Flat human dentin surfaces bonded with one of two mild self-etching adhesives, SE One (SE) or Scotchbond Universal (SU) applied either with scrubbing or without scrubbing technique, were prepared (n = 5). The microtensile bond strengths (μTBS), SE micrographs of morphological changes on treated dentin surfaces, and expression of nanoleakage along the bonded dentin interfaces as shown with TEM were evaluated. μTBS data were analyzed using two-way ANOVA and the post-hoc t-test at the significance level of 0.05.
    RESULTS: The scrubbing technique had a significant positive effect on the μTBS of SU (p < 0.05), while it produced no significant difference for SE (p > 0.05). Morphological evaluation of the treated dentin surfaces demonstrated that SU with scrubbing showed the highest etching ability, followed by scrubbing SE > nonscrubbing SE > nonscrubbing SU. In the nonscrubbing groups, nanoleakage formation using SU exhibited a reticular pattern throughout the hybridized complex, whereas with SE, water-tree nanoleakage was only found in the adhesive layer at dentinal tubule orifices. The scrubbing groups of both adhesives did not exhibit any nanoleakage expression.
    CONCLUSION: Using a scrubbing technique when applying mild self-etching adhesives could improve resin monomer infiltration into dentin, chase water on adhesive surfaces, and facilitate smear layer removal.

    (27163111) – as supplied by publisher]

    Bibliography

  • Fractography of interface after microtensile bond strength test using swept-source optical coherence tomography.
    .

    Fractography of interface after microtensile bond strength test using swept-source optical coherence tomography.

    Dent Mater. 2016 Apr 11;

    Authors: Dao Luong MN, Shimada Y, Turkistani A, Tagami J, Sumi Y, Sadr A

    Abstract
    OBJECTIVE: To determine the effect of crosshead speed and placement technique on interfacial crack formation in microtensile bond strength (MTBS) test using swept-source optical coherence tomography (SS-OCT).
    MATERIALS AND METHODS: MTBS test beams (0.9×0.9mm(2)) were prepared from flat human dentin disks bonded with self-etch adhesive (Clearfil SE Bond, Kuraray) and universal composite (Clearfil AP-X, Kuraray) with or without flowable composite lining (Estelite Flow Quick, Tokuyama). Each beam was scanned under SS-OCT (Santec, Japan) at 1319nm center wavelength before MTBS test was performed at crosshead speed of either 1 or 10mm/min (n=10). The beams were scanned by SS-OCT again to detect and measure cracks at the debonded interface using digital image analysis software. Representative beams were observed under confocal laser scanning microscope to confirm the fractography findings.
    RESULTS: Two-way ANOVA showed that for MTBS the crosshead speed was not a significant factor (p>0.05), while there was a difference between placement techniques (p<0.001), with flowable lining yielding higher mean values. On the other hand, for crack formation, there was a significant difference between crosshead speeds (p<0.01), while the placement technique did not show up as a statistically significant factor (p>0.05). The interaction of factors were not significant (p>0.05).
    SIGNIFICANCE: Testing MTBS samples at higher crosshead speeds induced more cracks in dentin. Lining with a flowable composite improved the bonding quality and increased the bond strength. SS-OCT can visualize interfacial cracks after restoration debonding.

    (27080369) – as supplied by publisher]

    Bibliography

  • Effects of coating materials on nanoindentation hardness of enamel and adjacent areas.

    Effects of coating materials on nanoindentation hardness of enamel and adjacent areas.

    Dent Mater. 2016 Apr 7;

    Authors: Alsayed EZ, Hariri I, Nakashima S, Shimada Y, Bakhsh TA, Tagami J, Sadr A

    Abstract
    OBJECTIVES: Materials that can be applied as thin coatings and actively release fluoride or other bioavailable ions for reinforcing dental hard tissue deserve further investigation. In this study we assessed the potential of resin coating materials in protection of underlying and adjacent enamel against demineralization challenge using nanoindentation.
    METHODS: Enamel was coated using Giomer (PRG Barrier Coat, PBC), resin-modified glass-ionomer (Clinpro XT Varnish, CXT), two-step self-etch adhesive (Clearfil SE Protect, SEP) or no coating (control). After 5000 thermal cycles and one-week demineralization challenge, Martens hardness of enamel beneath the coating, uncoated area and intermediate areas was measured using a Berkovich tip under 2mN load up to 200μm depth. Integrated hardness and 10-μm surface zone hardness were compared among groups.
    RESULTS: Nanoindentation and scanning electron microscopy suggested that all materials effectively prevented demineralization in coated area. Uncoated areas presented different hardness trends; PBC showed a remarkable peak at the surface zone before reaching as low as the control, while CXT showed relatively high hardness values at all depths.
    SIGNIFICANCE: Ion-release from coating materials affects different layers of enamel. Coatings with fluoride-releasing glass fillers contributed to reinforcement of adjacent enamel. Surface prereacted glass filler-containing PBC superficially protected neighboring enamel against demineralization, while resin-modified glass-ionomer with calcium (CXT) improved in-depth protection. Cross-sectional hardness mapping of enamel on a wide range of locations revealed minute differences in its structure.

    (27063457) – as supplied by publisher]

    Bibliography

  • In vitro dentine remineralization with a potential salivary phosphoprotein homologue.

    In vitro dentine remineralization with a potential salivary phosphoprotein homologue.

    Arch Oral Biol. 2016 Mar 23;68:35-42

    Authors: Romero MJ, Nakashima S, Nikaido T, Sadr A, Tagami J

    Abstract
    OBJECTIVE: Advantages of introducing a salivary phosphoprotein homologue under standardized in vitro conditions to simulate the mineral-stabilizing properties of saliva have been proposed. This study longitudinally investigates the effects of casein, incorporated as a potential salivary phosphoprotein homologue in artificial saliva (AS) solutions with/without fluoride (F) on in vitro dentine lesion remineralization.
    DESIGN: Thin sections of bovine root dentine were demineralized and allocated randomly into 6 groups (n=18) having equivalent mineral loss (ΔZ) after transverse microradiography (TMR). The specimens were remineralized using AS solutions containing casein 0μg/ml, F 0ppm (C0-F0); casein 0μg/ml, F 1ppm (C0-F1); casein 10μg/ml, F 0ppm (C10-F0); casein 10μg/ml, F 1ppm (C10-F1); casein 100μg/ml, F 0ppm (C100-F0) or casein 100μg/ml, F 1ppm (C100-F1) for 28days with TMR taken every 7 days.
    RESULTS: Surface mineral precipitation, evident in group C0-F1, was apparently inhibited in groups with casein incorporation. Repeated measures ANOVA with Bonferroni correction revealed higher ΔZ for non-F and non-casein groups than for their counterparts (p<0.001). Subsequent multiple comparisons showed that mineral gain was higher (p<0.001) with 10μg/ml casein than with 100μg/ml when F was present in the earlier stages of remineralization, with both groups achieving almost complete remineralization after 28 days.
    CONCLUSION: Casein is a potential salivary phosphoprotein homologue that could be employed for in vitro dentine remineralization studies. Concentration related effects may be clinically significant and thus must be further examined.

    (27054701) – as supplied by publisher]

    Bibliography

  • In vitro dentine remineralization with a potential salivary phosphoprotein homologue.

    In vitro dentine remineralization with a potential salivary phosphoprotein homologue.

    Arch Oral Biol. 2016 Mar 23;68:35-42

    Authors: Romero MJ, Nakashima S, Nikaido T, Sadr A, Tagami J

    Abstract
    OBJECTIVE: Advantages of introducing a salivary phosphoprotein homologue under standardized in vitro conditions to simulate the mineral-stabilizing properties of saliva have been proposed. This study longitudinally investigates the effects of casein, incorporated as a potential salivary phosphoprotein homologue in artificial saliva (AS) solutions with/without fluoride (F) on in vitro dentine lesion remineralization.
    DESIGN: Thin sections of bovine root dentine were demineralized and allocated randomly into 6 groups (n=18) having equivalent mineral loss (ΔZ) after transverse microradiography (TMR). The specimens were remineralized using AS solutions containing casein 0μg/ml, F 0ppm (C0-F0); casein 0μg/ml, F 1ppm (C0-F1); casein 10μg/ml, F 0ppm (C10-F0); casein 10μg/ml, F 1ppm (C10-F1); casein 100μg/ml, F 0ppm (C100-F0) or casein 100μg/ml, F 1ppm (C100-F1) for 28days with TMR taken every 7 days.
    RESULTS: Surface mineral precipitation, evident in group C0-F1, was apparently inhibited in groups with casein incorporation. Repeated measures ANOVA with Bonferroni correction revealed higher ΔZ for non-F and non-casein groups than for their counterparts (p<0.001). Subsequent multiple comparisons showed that mineral gain was higher (p<0.001) with 10μg/ml casein than with 100μg/ml when F was present in the earlier stages of remineralization, with both groups achieving almost complete remineralization after 28 days.
    CONCLUSION: Casein is a potential salivary phosphoprotein homologue that could be employed for in vitro dentine remineralization studies. Concentration related effects may be clinically significant and thus must be further examined.

    (27054701) – as supplied by publisher]

    Bibliography

  • Assessment of cervical demineralization induced by Streptococcus mutans using swept-source optical coherence tomography.

    Assessment of cervical demineralization induced by Streptococcus mutans using swept-source optical coherence tomography.

    J Med Imaging (Bellingham). 2016 Jan;3(1):014504

    Authors: Tezuka H, Shimada Y, Matin K, Ikeda M, Sadr A, Sumi Y, Tagami J

    Abstract
    Exposed root surfaces due to gingival recession are subject to biofilm stagnation that can result in caries formation. Cervical enamel and dentin demineralization induced by a cariogenic biofilm was evaluated using swept-source optical coherence tomography (SS-OCT). The cementoenamel junction (CEJ) sections of extracted human teeth were subjected to demineralization for 1, 2, or 3 weeks. A suspension of Streptococcus mutans was applied to form a cariogenic biofilm using an oral biofilm reactor. After incubation, demineralization was observed by SS-OCT. For the analysis of SS-OCT signal, the value of the area under the curve (AUC) of the signal profile was measured. Statistical analyses were performed with 95% level of confidence. Cervical demineralization was displayed as a bright zone in SS-OCT. The demineralization depth of dentin was significantly deeper than that of enamel ([Formula: see text]). Enamel near the CEJ demonstrated a significant increase of AUC over the other enamel region after the demineralization. The gaps along the dentinoenamel junction were additionally observed in SS-OCT. SS-OCT was capable of monitoring the cervical demineralization induced by a cariogenic biofilm and is considered to be a promising modality for the diagnosis of cervical demineralization.

    (27014718)]

    Bibliography

  • Adsorption behavior of methacryloyloxydecyl dihydrogen phosphate on an apatite surface at neutral pH.
    .

    Adsorption behavior of methacryloyloxydecyl dihydrogen phosphate on an apatite surface at neutral pH.

    Eur J Oral Sci. 2016 Mar 2;

    Authors: Bista B, Nakashima S, Nikaido T, Sadr A, Takagaki T, Romero MJ, Sato T, Tagami J

    Abstract
    This study aimed to quantify the adsorption affinity of neutralized 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP-N) toward hydroxyapatite (HA) and dicalcium phosphate dihydrate (DCPD) at pH 7.0 by employing the Langmuir isotherm model. Furthermore, the effects of inorganic phosphate (Pi) and fluoride (F(-) ) ions on the adsorption of 10-MDP-N onto HA and DCPD were examined. Fixed amounts of HA and DCPD powders were suspended in different concentrations of 10-MDP-N solutions and were incubated for 18 h. Equilibrated concentrations of 10-MDP-N were measured by spectrophotometry and the adsorption affinity was estimated using the Langmuir model. Moreover, the adsorption was examined by zeta-potential analysis. The results indicated that significant Langmuir correlation was noted in both substrates, along with an increasing negative zeta-potential; however, in DCPD the correlation was less strong. The addition of 1.0 mM Pi slightly delayed the adsorption of 10-MDP-N onto both substrates, whereas 3.0 mM Pi drastically delayed adsorption onto HA but completely inhibited adsorption onto DCPD. Up to 50 ppm, F(-) enhanced the adsorption onto HA, and the adsorption plateaued at higher concentrations of F(-) , whereas no obvious influence of F(-) on the adsorption onto DCPD was noted.

    (26932315) – as supplied by publisher]

    Bibliography

  • Adsorption behavior of methacryloyloxydecyl dihydrogen phosphate on an apatite surface at neutral pH.
    .

    Adsorption behavior of methacryloyloxydecyl dihydrogen phosphate on an apatite surface at neutral pH.

    Eur J Oral Sci. 2016 Mar 2;

    Authors: Bista B, Nakashima S, Nikaido T, Sadr A, Takagaki T, Romero MJ, Sato T, Tagami J

    Abstract
    This study aimed to quantify the adsorption affinity of neutralized 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP-N) toward hydroxyapatite (HA) and dicalcium phosphate dihydrate (DCPD) at pH 7.0 by employing the Langmuir isotherm model. Furthermore, the effects of inorganic phosphate (Pi) and fluoride (F(-) ) ions on the adsorption of 10-MDP-N onto HA and DCPD were examined. Fixed amounts of HA and DCPD powders were suspended in different concentrations of 10-MDP-N solutions and were incubated for 18 h. Equilibrated concentrations of 10-MDP-N were measured by spectrophotometry and the adsorption affinity was estimated using the Langmuir model. Moreover, the adsorption was examined by zeta-potential analysis. The results indicated that significant Langmuir correlation was noted in both substrates, along with an increasing negative zeta-potential; however, in DCPD the correlation was less strong. The addition of 1.0 mM Pi slightly delayed the adsorption of 10-MDP-N onto both substrates, whereas 3.0 mM Pi drastically delayed adsorption onto HA but completely inhibited adsorption onto DCPD. Up to 50 ppm, F(-) enhanced the adsorption onto HA, and the adsorption plateaued at higher concentrations of F(-) , whereas no obvious influence of F(-) on the adsorption onto DCPD was noted.

    (26932315) – as supplied by publisher]

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