↑ Return to Publications

Print this Page

Papers

  • In situ characterization of resin-dentin interfaces using conventional vs. cryofocused ion-beam milling.
    .

    In situ characterization of resin-dentin interfaces using conventional vs. cryofocused ion-beam milling.

    Dent Mater. 2015 May 15;

    Authors: Bakhsh TA, Sadr A, Mandurah MM, Shimada Y, Zakaria O, Tagami J

    Abstract
    OBJECTIVE: The introduction of focused ion beam (FIB) milling has facilitated preparation of hard tissue samples for transmission electron microscope (TEM). However, this technique generates high temperature that may alter or damage morphological features in biological tissue. Therefore, the aim of this study was to determine the effects of cryogenic cooling on the morphological features of dentin interfaces with dental restorative materials in samples prepared by FIB for TEM examination.
    METHODS: After preparation of a cylindrical-shaped cavities in extracted, non-carious premolar teeth, the specimens were restored with dental adhesive/composite and categorized into two restorative materials groups; (PB) a combination of Clearfil Protect Bond (Kuraray Noritake Dental, Japan)/Estelite Sigma Quick composite (Tokuyama Dental, Japan), and (SB) Filtek Silorane restorative system (3M ESPE, USA). The specimens were subjected to interfacial cross-sectioning, followed by observation and area selection using confocal laser microscopy. Later, ultrathin sections were prepared using FIB with cryogenic cooling (PB-C) and (SB-C), or without cooling (PB-NC) and (SB-NC) that all were examined under TEM.
    RESULTS: Resulting TEM images of the ultra-morphological features at the resin-dentin nano-interaction zone were improved when FIB preparation was conducted in the cryogenic condition and no sign of artifacts were detected.
    SIGNIFICANCE: Conducting ion beam milling with cryogenic cooling was advantageous in minimizing the elevation in specimen temperature. This led to preservation of dentin microstructures that revealed additional information about substrates that are necessary for advanced characterization of tooth-biomaterial interactions.

    (25986333) – as supplied by publisher]

    Bibliography

  • In situ characterization of resin-dentin interfaces using conventional vs. cryofocused ion-beam milling.
    .

    In situ characterization of resin-dentin interfaces using conventional vs. cryofocused ion-beam milling.

    Dent Mater. 2015 May 15;

    Authors: Bakhsh TA, Sadr A, Mandurah MM, Shimada Y, Zakaria O, Tagami J

    Abstract
    OBJECTIVE: The introduction of focused ion beam (FIB) milling has facilitated preparation of hard tissue samples for transmission electron microscope (TEM). However, this technique generates high temperature that may alter or damage morphological features in biological tissue. Therefore, the aim of this study was to determine the effects of cryogenic cooling on the morphological features of dentin interfaces with dental restorative materials in samples prepared by FIB for TEM examination.
    METHODS: After preparation of a cylindrical-shaped cavities in extracted, non-carious premolar teeth, the specimens were restored with dental adhesive/composite and categorized into two restorative materials groups; (PB) a combination of Clearfil Protect Bond (Kuraray Noritake Dental, Japan)/Estelite Sigma Quick composite (Tokuyama Dental, Japan), and (SB) Filtek Silorane restorative system (3M ESPE, USA). The specimens were subjected to interfacial cross-sectioning, followed by observation and area selection using confocal laser microscopy. Later, ultrathin sections were prepared using FIB with cryogenic cooling (PB-C) and (SB-C), or without cooling (PB-NC) and (SB-NC) that all were examined under TEM.
    RESULTS: Resulting TEM images of the ultra-morphological features at the resin-dentin nano-interaction zone were improved when FIB preparation was conducted in the cryogenic condition and no sign of artifacts were detected.
    SIGNIFICANCE: Conducting ion beam milling with cryogenic cooling was advantageous in minimizing the elevation in specimen temperature. This led to preservation of dentin microstructures that revealed additional information about substrates that are necessary for advanced characterization of tooth-biomaterial interactions.

    (25986333) – as supplied by publisher]

    Bibliography

  • Evaluation of a new hardness tester (Cariotester): Comparison with transverse microradiography for assessing the inhibitory effect of fluoride application on bovine root dentin demineralization.
    .

    Evaluation of a new hardness tester (Cariotester): Comparison with transverse microradiography for assessing the inhibitory effect of fluoride application on bovine root dentin demineralization.

    Dent Mater J. 2015 May 1;

    Authors: Sugawara T, Nakashima S, Shimizu A, Tagami J, Momoi Y

    Abstract
    The objective of this study was to investigate the correlation between CT depth, indentation depth determined by a new hardness tester (Cariotester), and the transverse microradiography (TMR) parameters, i.e., lesion depth and mineral loss. For that purpose, this study evaluated the feasibility of using Cariotester as a root caries diagnostic system and capability of Cariotester to detect effect of fluoride application on inhibiting dentin demineralization. Fluorides were applied to bovine root dentin specimens, which were subsequently demineralized for 1-21 days and then CT depth and TMR parameters were assessed. There were significant correlations between CT depth and TMR parameters in fluoride and non-fluoride groups. There were significant differences between fluoride and non-fluoride groups for CT depth and TMR parameters respectively. Current results suggested that Cariotester may be capable of providing an objective evaluation of root caries progression and the fluoride effect on inhibiting dentin demineralization.

    (25948139) – as supplied by publisher]

    Bibliography

  • Validation of Optical Coherence Tomography against Micro-computed Tomography for Evaluation of Remaining Coronal Dentin Thickness.
    .

    Validation of Optical Coherence Tomography against Micro-computed Tomography for Evaluation of Remaining Coronal Dentin Thickness.

    J Endod. 2015 May 1;

    Authors: Majkut P, Sadr A, Shimada Y, Sumi Y, Tagami J

    Abstract
    INTRODUCTION: Optical coherence tomography (OCT) is a noninvasive modality to obtain in-depth images of biological structures. A dental OCT system has become available for chairside application. This in vitro study hypothesized that swept-source OCT can be used to measure the remaining dentin thickness (RDT) at the roof of the dental pulp chamber during excavation of deep caries.
    METHODS: Human molar teeth with deep occlusal caries were investigated. After obtaining 2-dimensional and 3-dimensional OCT scans using a swept-source OCT system at a 1330-nm center wavelength, RDT was evaluated by image analysis software. Microfocus x-ray computed tomographic (micro-CT) images were obtained from the same cross sections to confirm OCT findings. The smallest RDT values at the visible pulp horn were measured on OCT and micro-CT imaging and compared using the Pearson correlation. Pulpal horns and pulp chamber roof observation under OCT and micro-CT imaging resulted in comparable images that allowed the measurement of coronal dentin thickness.
    RESULTS: RDT measured by OCT showed optical values range between 140 and 2300 μm, which corresponded to the range of 92-1524 μm on micro-CT imaging. A strong correlation was found between the 2 techniques (r = 0.96, P < .001).
    CONCLUSIONS: Further analysis indicated linear regression with a slope of 1.54 and no intercept, closely matching the bulk refractive index of dentin. OCT enables visualization of anatomic structures during deep caries excavation. Exposure of the vital dental pulp because of the removal of very thin remaining coronal dentin can be avoided with this novel noninvasive technique.

    (25937180) – as supplied by publisher]

    Bibliography

  • Effect of phytic acid etchant on the structural stability of demineralized dentine and dentine bonding.
    .

    Effect of phytic acid etchant on the structural stability of demineralized dentine and dentine bonding.

    J Mech Behav Biomed Mater. 2015 Apr 6;48:145-152

    Authors: Kong K, Islam MS, Nassar M, Hiraishi N, Otsuki M, Yiu CK, Tagami J

    Abstract
    OBJECTIVE: This study examined the effect of phytic acid (IP6) in stabilizing the morphology of dentine collagen network and resin-dentine bonding.
    METHODS: Dentine beams were fully demineralized with 10% phosphoric acid (PA) or 1% IP6 (pH 1.2). PA-demineralized beams were divided into three groups: (a) no further treatment (control), (b) treatment with 5% glutaraldehyde (GA) for 1h and (c) treatment with 1% IP6 (pH 7) for 1h. IP6-demineralized beams received no further treatment. The beams were then subjected to ultimate tensile strength (UTS) testing. Dentine micromorphology evaluation was performed using a field-emission scanning electron microscope (FE-SEM). Dentine disks were etched with 35% PA for 15s or 1% IP6 for 30s. PA-etched dentine disks were divided into three groups as (a), (b) and (c) as for UTS testing, but the treatment with GA or IP6 was done in 1min. For microtensile bond strength (µTBS) testing, flat dentine surfaces etched with PA or IP6 were blot-dried (wet dentine) or air-dried for 10s (dry dentine) and bonded with an etch-and-rinse adhesive followed by composite build-up.
    RESULTS: IP6-demineralized dentine showed significantly higher UTS, when compared to PA-demineralized dentine. GA and IP6 significantly improved UTS of PA-demineralized dentine. FE-SEM observation revealed that dentine collagen network was preserved by GA and IP6. No significant difference in µTBS was found between the wet and dry IP6-etched dentine groups.
    CONCLUSION: IP6 etching showed a structural stabilizing effect on demineralized dentine matrix and produced good resin-dentine bonding, regardless of dentine moistness or dryness.

    (25933170) – as supplied by publisher]

    Bibliography

  • Assessment of natural enamel lesions with optical coherence tomography in comparison with microfocus x-ray computed tomography

    Assessment of natural enamel lesions with optical coherence tomography in comparison with microfocus x-ray computed tomography

    Espigares J, Sadr A, Hamba H, Shimada Y, Otsuki M, Tagami J, Sumi Y.

    J. Med. Imag. 2(1), 014001 (Feb 11, 2015). doi:10.1117/1.JMI.2.1.014001

    A technology to characterize early enamel lesions is needed in dentistry. Optical coherence tomography (OCT) is a noninvasive method that provides high-resolution cross-sectional images. The aim of this study is to compare OCT with microfocus x-ray computed tomography (μCT ) for assessment of natural enamel lesions in vitro. Ten human teeth with visible white spot-like changes on the enamel smooth surface and no cavitation (ICDAS code 2) were subjected to imaging by μCT (SMX-100CT, Shimadzu) and 1300-nm swept-source OCT (Dental SS-OCT, Panasonic Health Care). In μCT , the lesions appeared as radiolucent dark areas, while in SS-OCT, they appeared as areas of increased signal intensity beneath the surface. An SS-OCT attenuation coefficient based on Beer–Lambert law could discriminate lesions from sound enamel. Lesion depth ranged from 175 to 606  μm in SS-OCT. A correlation between μCT and SS-OCT was found regarding lesion depth (R=0.81 , p<0.001 ) and also surface layer thickness (R=0.76 , p<0.005 ). The images obtained clinically in real time using the dental SS-OCT system are suitable for the assessment of natural subsurface lesions and their surface layer, providing comparable images to a laboratory high-resolution μCT without the use of x-ray.

  • Optical coherence tomography for evaluation of enamel and protective coatings.
    .

    Optical coherence tomography for evaluation of enamel and protective coatings.

    Dent Mater J. 2015;34(1):98-107

    Authors: Alsayed EZ, Hariri I, Sadr A, Nakashima S, Bakhsh TA, Shimada Y, Sumi Y, Tagami J

    Abstract
    Optical coherence tomography (OCT) is an interferometric imaging technique. This study aimed to employ OCT to evaluate four different resin-based materials including a coating containing glass-ionomer filler and calcium, a giomer, and two fluoride-releasing self-etch resins. The coating and its underlying and adjacent enamel were monitored using swept-source OCT (center wavelength: 1330 nm) at baseline, after 5,000 thermal cycles, and after 1, 4 and 7 days of demineralization (pH 4.5). The coatings showed different thicknesses (60-250 micrometers) and various levels of structural and interfacial integrity. OCT could detect a demineralization inhibition zone adjacent to the edge of the fluoride- and calcium-releasing material. Localized demineralization was occasionally observed under thinner coatings. Protection of susceptible enamel surfaces by thin resin-based bioactive coatings provides protection from demineralization. OCT can be used to non-destructively monitor the integrity of such coatings, as well as enamel changes beneath and adjacent to them.

    (25748465) – in process]

    Bibliography

  • The role of MDP in a bonding resin of a two-step self-etching adhesive system.
    .

    The role of MDP in a bonding resin of a two-step self-etching adhesive system.

    Dent Mater J. 2015 Feb 24;

    Authors: Matsui N, Takagaki T, Sadr A, Ikeda M, Ichinose S, Nikaido T, Tagami J

    Abstract
    The purpose of this study was to evaluate the role of 10-methacryloyloxydecyl dihydrogen phosphate (MDP) contained in the bonding resin of a two-step self-etch adhesive system. An experimental adhesive (M0) containing MDP only in the primer, but not in the bonding resin was prepared. Clearfil SE Bond (MM) and M0 were compared in terms of microtensile bond strength to dentin, ultimate tensile strength of the bonding resin, and dentin-resin bonding interface morphology under SEM and TEM. The immediate µTBS values of MM significantly decreased after thermal cycles while M0 were stable even after 10,000 cycles. In the SEM observations, formation of erosion was observed beneath the acid-base resistant zone only in M0. The results suggested that MDP in the bonding resin of the two-step self-etching system; 1) improved the immediate bond strength, but caused reduction in long-term bond durability; 2) offered the advantages of acid-base resistance at the ABRZ forefront area.

    (25740167) – as supplied by publisher]

    Bibliography

  • Age-specific prevalence of erosive tooth wear by acidic diet and gastroesophageal reflux in Japan.
    .

    Age-specific prevalence of erosive tooth wear by acidic diet and gastroesophageal reflux in Japan.

    J Dent. 2015 Feb 12;

    Authors: Kitasako Y, Sasaki Y, Takagaki T, Sadr A, Tagami J

    Abstract
    OBJECTIVES: To evaluate the age-specific prevalence of erosive tooth wear in Japanese adults.
    METHODS: The study sample consisted of a total of 1,108 adults aged 15 to 89 yrs in Tokyo, Japan. The subjects were asked to complete a self-administered nutrition-related questionnaire. Two examiners evaluated tooth wear in a full-mouth recording, using a modified tooth wear index developed based on the Smith and Knight Tooth Wear index. Subjects who had frequent acid consumption or gastric reflux and at least one tooth with an initial enamel smooth surface wear were placed in an erosive wear positive group, and the rest of subjects were placed in the erosive wear negative group.
    RESULTS: The median (IQR) prevalence of erosion was 19.1 (1.8) at enamel level and 6.5 (3.7) with dentin exposure. There were statistical differences in prevalence of erosive wear among different age groups (p<0.05). Dietary habits found to be frequent in erosive wear positive group included acidic juices for younger subjects (15-39 yrs), and acidic fruits for older subjects (60-89 yrs). The prevalence of gastroesophageal reflux and eating disorder was 3.5%. A severe loss of dental tissue was observed on labial and incisal surfaces of anterior teeth in the erosive wear positive group.
    CONCLUSIONS: In the studied sample of Japanese adults, 26.1% had signs of erosive wear. Clinical significance: Erosive wear, in combination with abrasion and attrition, results in severe loss of tooth tissue. Frequent consumption of acidic fruits and drinks was significantly associated with erosive tooth wear at different age groups.

    (25684603) – as supplied by publisher]

    Bibliography

  • Self-etch adhesive systems: a literature review.
    .

    Self-etch adhesive systems: a literature review.

    Braz Dent J. 2015 Jan-Feb;26(1):3-10

    Authors: Giannini M, Makishi P, Ayres AP, Vermelho PM, Fronza BM, Nikaido T, Tagami J

    Abstract
    This paper presents the state of the art of self-etch adhesive systems. Four topics are shown in this review and included: the historic of this category of bonding agents, bonding mechanism, characteristics/properties and the formation of acid-base resistant zone at enamel/dentin-adhesive interfaces. Also, advantages regarding etch-and-rinse systems and classifications of self-etch adhesive systems according to the number of steps and acidity are addressed. Finally, issues like the potential durability and clinical importance are discussed. Self-etch adhesive systems are promising materials because they are easy to use, bond chemically to tooth structure and maintain the dentin hydroxyapatite, which is important for the durability of the bonding.

    (25672377) – in process]

    Bibliography

  • Formation and characterization of hypermineralized zone beneath dentine lesion body induced by topical fluoride in-vitro.

    Formation and characterization of hypermineralized zone beneath dentine lesion body induced by topical fluoride in-vitro.

    Arch Oral Biol. 2015 Jan 10;60(4):574-581

    Authors: Khunkar SJ, Utaka S, Hariri I, Sadr A, Ikeda M, Nakashima S, Nikaido T, Tagami J

    Abstract
    OBJECTIVE: This in-vitro study aimed to evaluate and characterize the hypermineralized zone (Hyper-zone) formed beneath the remineralized dentine lesion body by transverse microradiography (TMR), scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDS).
    DESIGN: Demineralized bovine dentine specimens were treated with fluoride solutions (APF, NaF) and remineralized for 2-4 weeks. Then thin sections were prepared to characterize the Hyper-zone by TMR, EDS. Fractured specimen surfaces were observed by SEM.
    RESULTS: TMR analysis revealed a higher mineral density at Hyper-zone than that of sound dentine (48vol%) ranging from 50 up to 61vol% and the thickness ranging from 197 to 344μm for 4-week specimens, while specimens without fluoride treatment did not show Hyper-zone. SEM pictures at Hyper-zone showed no evident crystal-like deposits in dentinal tubules and no notable difference when compared to that in sound dentine. EDS analysis demonstrated higher concentrations of Ca and P at Hyper-zone than those in sound dentine, which corresponded to the TMR profile, while the magnesium (Mg) concentration was low at this zone.
    CONCLUSIONS: Demineralized dentine lesions exposed to fluoride and remineralization treatments exhibited Hyper-zone beneath the lesion body, in which the mineral density was higher than that of sound dentine. Possible mechanism for the formation of Hyper-zone was discussed by assuming removal of mineral regulators such as Mg and other organic substances from sound dentine during de-/remineralization processes.

    (25616245) – as supplied by publisher]

    Bibliography

  • Immobilization of phosphate monomers on collagen induces biomimetic mineralization.
    .

    Immobilization of phosphate monomers on collagen induces biomimetic mineralization.

    Biomed Mater Eng. 2015 Jan 1;25(1):89-99

    Authors: Nurrohman H, Nakashima S, Takagaki T, Sadr A, Nikaido T, Asakawa Y, Uo M, Marshall SJ, Tagami J

    Abstract
    BACKGROUND: Immobilization of phosphoproteins on type-I collagen via covalent binding may induce extra- and intrafibrillar mineralization.
    OBJECTIVE: This study tested the hypothesis that methacrylate phosphate esters immobilized on reconstituted type-I collagen can mimic the nucleating role of phosphoproteins.
    METHODS: Three functional monomers (MDP, GPDM and Phenyl-P) that differed in chemical structure and steric hindrances around the phosphate moiety were evaluated. Reconstituted type-I collagen was either left untouched (control) or treated by 5% monomer/ethanol for 20 s. All samples were incubated in simulated dentinal fluid as mineralizing medium at 37°C for 7 or 14 days. The extra- and intrafibrillar mineralization were examined by SEM and TEM/SAED crystallography, respectively.
    RESULTS: FT-IR spectroscopy showed that the phosphate groups were incorporated on reconstituted collagen, irrespective of their chemical structure. MDP immobilization induced dense growth of extrafibrillar mineral over time, while with GPDM- and Phenyl-P-immobilized collagen, mineralization was moderate and sparse, respectively. TEM/SAED evidence disclosed that intrafibrillar minerals exclusively occurred in MDP-immobilized collagen.
    CONCLUSIONS: Immobilization of MDP, which had the lowest steric hindrance, could induce significant biomimetic extra- and intrafibrillar mineralization; resembling the lowest level of hierarchy organization of dentin.

    (25585983) – in process]

    Bibliography

  • Molecular level evaluation on HEMA interaction with a collagen model.

    Molecular level evaluation on HEMA interaction with a collagen model.

    Dent Mater. 2014 Dec 10;

    Authors: Hiraishi N, Tochio N, Kigawa T, Otsuki M, Tagami J

    Abstract
    OBJECTIVE: 2-Hydroxyethylmethacrylate (HEMA) diffuses in wet dentin and promotes adhesion during dentin priming and bonding. We have investigated the molecular level interaction between HEMA and a collagen model by using saturation transfer difference (STD) NMR.
    METHODS: The binding of HEMA to collagen was preliminarily investigated by suspending demineralized human dentin powders in a 4mM HEMA solution for 1h and measuring the decrease in the HEMA concentration on a spectrophotometer. The molecular level interaction of HEMA with atelocollagen, which was used as a collagen model, was investigated by STD-NMR spectroscopy.
    RESULTS: The HEMA concentration in the suspension did not change, indicating that HEMA did not bind to dentin collagen. This was confirmed by STD-NMR; when the atelocollagen resonance was saturated, no saturation was propagated to HEMA and no STD signals were detected.
    SIGNIFICANCE: The HEMA protons were not near the atelocollagen surface, indicating HEMA did not interact with atelocollagen. The collagen fibrils may be surrounded by water molecules in dentin/bond interfaces, which prevent the direct HEMA binding interaction.

    (25499247) – as supplied by publisher]

    Bibliography

  • Phytic Acid: An Alternative Root Canal Chelating Agent.
    .

    Phytic Acid: An Alternative Root Canal Chelating Agent.

    J Endod. 2014 Nov 20;

    Authors: Nassar M, Hiraishi N, Tamura Y, Otsuki M, Aoki K, Tagami J

    Abstract
    INTRODUCTION: The objectives of this study were to investigate the effect of phytic acid, inositol hexakisphosphate (IP6), as a final rinse on the surface of instrumented root canals and smear-layered flat dentin surfaces treated with sodium hypochlorite (NaOCl) and to evaluate its effect on the viability and alkaline phosphatase activity of osteoblast-like cells (MC3T3-E1).
    METHODS: The universally accepted chelating agent EDTA was used as the control in all conducted experiments. Root canals of human canines were instrumented with rotary files and irrigated with 5% NaOCl, followed by a final rinse of 17% EDTA (1 minute), 1% IP6 (1 minute or 30 seconds), or distilled water. NaOCl-treated flat coronal dentin surfaces were also treated with 17% EDTA (1 minute), 1% IP6 (1 minute or 30 seconds), or distilled water. The presence or absence of smear layer was evaluated with scanning electron microscopy. Cell viability and alkaline phosphatase assays were performed to evaluate the effect of IP6 and EDTA on cultured MC3T3-E1 cells.
    RESULTS: The results demonstrated the ability of IP6 to remove the smear layer from instrumented root canals and flat coronal dentin surfaces. When compared with EDTA, IP6 was less cytotoxic and did not affect the differentiation of MC3T3-E1 cells.
    CONCLUSIONS: IP6 shows the potential to be an effective and biocompatible chelating agent.

    (25453568) – as supplied by publisher]

    Bibliography

  • Effect of different desensitizers on inhibition of bovine dentin demineralization: micro-computed tomography assessment.
      .

    Effect of different desensitizers on inhibition of bovine dentin demineralization: micro-computed tomography assessment.

    Eur J Oral Sci. 2014 Nov 1;

    Authors: Lodha E, Hamba H, Nakashima S, Sadr A, Nikaido T, Tagami J

    Abstract
    This study evaluated the effect of two desensitizers on inhibition of dentin demineralization, after immersion in artificial saliva using micro-computed tomography (μCT). Dentin blocks cut from bovine incisors were treated with deionized water (DW, a negative control) or one of three desensitizers: a fluoride varnish (Duraphat, a positive control), a calcium phosphate desensitizer (Teethmate Desensitizer), and a fluoro-alumino-calcium silicate-based desensitizer (Nanoseal). After each treatment, the specimens in Duraphat, Nanoseal, and Teethmate Desensitizer groups were pre-immersed in artificial saliva (pH 6.5) for either 1 d or 1 wk. The mineral loss of the specimens after demineralization (pH 5.0, 3 h) was evaluated by μCT. The treated surface was investigated with scanning electron microscopy. Mineral loss in all treatment groups was significantly lower than that in DW. Duraphat was the most effective treatment against demineralization, followed by Nanoseal. Nanoseal showed significantly better reduction in mineral loss following immersion for 1 wk in artificial saliva than for 1 d. However, Teethmate Desensitizer and Duraphat did not exhibit enhanced inhibition of demineralization over a longer period of immersion in artificial saliva. Scanning electron microscopy images showed deposition of particles on the dentin in both Teethmate Desensitizer. The application of Teethmate Desensitizer and Nanoseal to the exposed dentin surface resulted in inhibition of demineralization, with Nanoseal resulting in improved inhibition after prolonged immersion in artificial saliva.

    (25363830)

    Bibliography

  • Ultramorphological Assessment of Dentin-Resin Interface After Use of Simplified Adhesives.
    .

    Ultramorphological Assessment of Dentin-Resin Interface After Use of Simplified Adhesives.

    Oper Dent. 2014 Oct 9;

    Authors: Marghalani H, Bakhsh T, Sadr A, Tagami J

    Abstract
    SUMMARY This study assessed dentin/resin interface integration in Class I cavities restored with simplified adhesives by using a focused ion-beam milling (FIB) and transmission electron microscope (TEM). Class I cavities (1.5-mm depth with dentin thickness of ∼0.5 mm, 4-mm length, and 2-mm width) were prepared on freshly extracted, sound human molars. Two all-in-one adhesive systems (Scotchbond/Single Bond Universal [SUD] and Xeno-V(+) [X5D]) were used and compared with a two-step etch-and-rinse system (Prime&Bond NT [NTD]). The adhesives were applied according to the manufacturers’ guidelines. A universal resin composite (Filtek Z350 XT Universal) was used to restore the cavities in one bulk filling and was irradiated at 550 mW/cm(2) for 40 seconds by a quartz-tungsten-halogen light (Optilux 501). After exposure to liquid nitrogen coolant, the specimens were milled to nanoscale thickness by FIB to view and then assess the area of dentin-resin interface by TEM. Unlike the unfilled X5D, a noticeably smooth transition zone at the dentin-resin interface was shown for the SUD and NTD adhesives. The SUD demonstrated an uneven hybrid layer with clearly demineralized collagen bundles. Ultramorphologically, dispersed needlelike apatite crystals were detected within the partially demineralized dentin or the hybrid layer of both compositionally different all-in-one simplified adhesives. Conversely, these crystals were entirely absent from the hybrid layer of the etch-and-rinse NTD adhesive. In the X5D group, a bright band was noted beneath the hybrid layer. The methacryloxydecyl dihydrogen phosphate monomer containing ultramild self-etch adhesive (SUD) was still validated in terms of its capability in dentin adhesion.

    (25299704)- as supplied by publisher]

    Bibliography

  • The effect of tooth age on color adjustment potential of resin composite restorations.
    .

    The effect of tooth age on color adjustment potential of resin composite restorations.

    J Dent. 2014 Sep 18;

    Authors: Tanaka A, Nakajima M, Seki N, Foxton R, Tagami J

    Abstract
    OBJECTIVES: The purpose of this study was to investigate the effect of tooth age on color adjustment potential of resin composite restorations in human teeth.
    METHODS: Twenty extracted human premolars with an A2 shade, extracted for orthodontic reasons from younger patients (20-28 yrs) (younger teeth) and periodontal reasons from older patients (45-69 yrs) (older teeth), were used in this study. Cylindrical shaped cavities (3.0mm depth; 2.0mm diameter) were prepared in the center of the crowns on the buccal surface. One of four resin composites of A2 shade (Kalore, KA; Solare, SO; Clearfil Majesty, MJ; Beautifil II, BF) was placed in the cavity, and the color was measured at four areas (0.4mm x 0.4 mm(2)) on the restored teeth (area 1; tooth area 1.0mm away from the border of resin composite restoration: area 2; tooth border area 0.3mm away from margin of resin composite restoration: area 3; resin composite border area 0.3mm away from margin of resin composite restoration: area 4; resin composite area at the center of resin composite restoration) using a spectrophotometer (Crystaleye). The color of each area was determined according to the CIELAB color scale. Color differences (ΔE*) between the areas of 1 and 2, 2 and 3, 3 and 4 and 1 and 4 were calculated, and also the ratio of ΔE*area2-3 to ΔE*area1-4 (ΔE*area2-3/1-4), ΔE*area3-4 to ΔE*area1-4 (ΔE*area3-4/1-4) and ΔE*area1-2 to ΔE*area1-4 (ΔE*area1-2/1-4) as a parameter of the color shift in resin composite restoration, were determined. Moreover, the light transmission characteristics of the resin materials and dentine discs from the younger and older teeth were measured using a goniophotometer. The data were statistically analyzed using two-way ANOVA, and Dunnett’s T3 and t-test for the post-hoc test.
    RESULTS: ΔE*area2-3 (color difference between resin composite and tooth at the border) and ΔE*area1-4 (color difference between resin composite and tooth) of the older teeth groups were significantly larger than those of younger teeth groups (p<0.05). The ΔE*area2-3/1-4 (mis-match rate in color shifting at the border) of the older teeth groups was larger than that of the younger teeth groups (p<0.05). ΔE*area3-4/1-4 (color shifting rate of resin composite side) was significantly larger in older teeth than younger teeth (p<0.05), while ΔE*area1-2/1-4 (color shifting rate of tooth side), was significantly smaller in older teeth than younger teeth (p<0.05). In each tooth group, there were no significant differences in ΔE*area2-3, ΔE*area1-4, ΔE*area2-3/1-4, ΔE*area3-4/1-4 and ΔE*area1-2/1-4 between the materials (p>0.05). Analysis of the light transmission properties indicated that older dentine transmitted more light, while younger dentine exhibited greater light diffusion and transmitted less light.
    CONCLUSIONS: The color shifting effects at the border of the resin composite restorations were influenced by the age of the tooth. This behavior might be influenced by the light transmission characteristics of dentine in restored teeth. Clinical Significance: The potential for color adjustment of resin composite restorations may be less in older teeth than younger teeth.

    (25242100)- as supplied by publisher]

    Bibliography

  • Effect of hesperidin incorporation into a self-etching primer on durability of dentin bond.
    .

    Effect of hesperidin incorporation into a self-etching primer on durability of dentin bond.

    Dent Mater. 2014 Sep 3;

    Authors: Islam MS, Hiraishi N, Nassar M, Yiu C, Otsuki M, Tagami J

    Abstract
    OBJECTIVE: Collagen degradation at the resin-dentin interface deteriorates dentin bond durability. The use of natural cross-linkers might offer a positive approach to stabilize the resin-dentin interface. This study evaluated the effects of incorporation of natural cross-linkers into a self-etch adhesive primer on the immediate and long-term micro-tensile bond strengths (μTBS) to dentin.
    METHODS: Experimental primers were prepared by incorporating either 0.5%, 1%, 2%, 5% of hesperidin (HPN) or 0.5% of proanthocyanidins (PA) into Clearfil SE primer. Extracted human molar teeth were restored using the experimental primers or the pure SE primer (control). The mechanical properties of the bonded interfaces were measured using the nano-indentation tests. Beam-shaped bonded specimens were sub-divided for one-day and one-year μTBS test. Interfacial collagen morphology was observed using transmission electron microscopy.
    RESULT: The immediate μTBS significantly increased in 0.5%, 1% and 2% HPN-incorporated groups when compared with the control. The mechanical properties of bonded interface were improved with 1% and 2% HPN-incorporated primers. For the long-term μTBS, the 2% and 5% HPN-incorporated group were significantly higher than the control. The morphology of the collagen fibrils were preserved by 5% HPN-incorporation after one-year storage. The PA group, however, failed to improve the μTBS and the mechanical properties of the bonded interfaces.
    SIGNIFICANCE: The incorporation of 2% HPN into the self-etching primer had a positive effect on the immediate μTBS and mechanical properties of the resin-dentin interfaces. The 5% HPN group preserved the morphology of the collagen in the hybrid layer after one-year storage in artificial saliva.

    (25194169)- as supplied by publisher]

    Bibliography

  • Effects of light curing method and resin composite composition on composite adaptation to the cavity wall.
    .

    Effects of light curing method and resin composite composition on composite adaptation to the cavity wall.

    Dent Mater J. 2014 Jul 2;

    Authors: Yoshikawa T, Morigami M, Sadr A, Tagami J

    Abstract
    This study aimed to evaluate the effects of the light curing method and resin composite composition on marginal sealing and resin composite adaptation to the cavity wall. Cylindrical cavities were prepared on the buccal or lingual cervical regions. The teeth were restored using Clearfil Liner Bond 2V adhesive system and filled with Clearfil Photo Bright or Palfique Estelite resin composite. The resins were cured using the conventional or slow-start light curing method. After thermal cycling, the specimens were subjected to a dye penetration test. The slow-start curing method showed better resin composite adaptation to the cavity wall for both composites. Furthermore, the slow-start curing method resulted in significantly improved dentin marginal sealing compared with the conventional method for Clearfil Photo Bright. The light-cured resin composite, which exhibited increased contrast ratios duringpolymerization, seems to suggest high compensation for polymerization contraction stress when using the slow-start curing method.

    (24988883)- as supplied by publisher]

    Bibliography

  • Sealing performance of resin cements before and after thermal cycling: evaluation by optical coherence tomography.
    .

    Sealing performance of resin cements before and after thermal cycling: evaluation by optical coherence tomography.

    Dent Mater. 2014 Sep;30(9):993-1004

    Authors: Turkistani A, Sadr A, Shimada Y, Nikaido T, Sumi Y, Tagami J

    Abstract

    OBJECTIVES: Self-adhesive resin cements have been recently introduced; however, there is little data available on their long-term performance. In this in vitro study, swept-source optical coherence tomography (OCT) at 1310 nm center wavelength was used for monitoring adaptation of indirect resin restorations after thermal cycling.

    METHODS: Resin inlays were luted to class-I cavities of extracted human teeth using three resin cements; Clearfil SA Luting (SA; Kuraray), Bistite II DC or Multibond II (Tokuyama Dental). Each cement was applied with or without pre-coating of dentin by a self-etch adhesive (Clearfil SE Bond) and a low-viscosity microfilled resin. OCT imaging was performed after 24 h, after 2000 and after 10,000 thermocycles (n=5). Selected samples were sectioned for interfacial observation by confocal laser scanning microscope (CLSM). Floor adaptation (percentage) was analyzed by software on 20 B-scans throughout each specimen, and subjected to statistical analysis by three-way ANOVA test at a significance level of 0.05.

    RESULTS: Resin cement type, resin coating and thermal aging all significantly affected adaptation (p<0.05). Initially, SA showed the highest adaptation; however, thermal aging significantly affected its sealing. The best results for all the cements were consistently achieved when the resin coating technique was applied where no deterioration of interfacial integrity was observed in the coated groups. CLSM closely confirmed OCT findings in all groups.

    SIGNIFICANCE: OCT could be used for monitoring of composite inlays with several interfacial resin layers. The application of a direct bonding agent in the resin-coating technique improved interfacial sealing and durability of all resin cements.

    (24946983) – in process]

    Bibliography